Regulation  Nutrition  Intestinal Flora  Detoxication  Food Additives  Probiotics  SBO


Soil based Organisms (SBOs)

In contrast to the well-known lactic acid bacteria generally available in yoghurt (Bifido and Lacto), the Dolgalet Series probiotics contain the soil based organisms (SBOs) playing an important role in nature, organic growth, nutrition and digestive organs of humans and animals. Only these micro organisms, often remaining underestimated, can transform inanimate minerals into organic, "alive" complexes of biologically active substances. To remain alive, humans and animals have to permanently assimilate life.

Probiotic soil bacteria form an integral part of balanced nutrition. Large quantities of these bacteria are present in unprocessed fruit and vegetables. Since time immemorial, they have been the obligatory intestinal inhabitants; digestive function and nutrients assimilation would have been impossible without their participation. Proper nutrition ensures sufficient consumption of probiotic soil bacteria - first of all, the bacteria of plant origin - with food. Share of probiotic soil bacteria studies (including those of the use of food additives) in the whole range of current intensive studies of the mechanisms of bacterial effects on human body has been gradually increasing. As soon as they enter the intestinal tract, they multiply and act as a bioreactor that produces enzymes, amino acids, vitamins and bacteriocins neutralizing pathogenic micro organisms without creating resistance or side effects. They clean the intestinal walls and increase their permeability for appropriate nutrients, and they restore the biological balance of intestinal flora.

Bacillus subtilis and Bacillus licheniformis bacteria contained in Dolgalet Series probiotics are globally known Gram-positive bacterial strains categorized as totally harmless eubacteria. Stick-like Bacillus subtilis bacteria measuring 2 to 4 micrometers in length are the most common soil bacteria. Because they are readily enriched in hay extract, they are also called as “hay bacillus”. Both rhizosphere and the upper strata of soil are inhabited by these bacteria. In view of their saprophytic mode of life (i.e., their ability to use inanimate organic material as food as well), they take part in the mobilization and mineralization of organic substances, as well as the return of organic substances in the nutrient cycle. In the rhizosphere, Bacillus subtilis prevents the growth of fungi by displacing them and protects plant roots from harmful micro organisms. In its natural environment, Bacillus subtilis is exposed to the effects of numerous biotic and abiotic factors. Virus infections, heating, hyperosmotic and hypoosmotic stresses, as well as the stresses related to heavy metals, insufficient nutritional and energy sources, restricted oxygen delivery, changed pH levels, chemical radicals, etc., and the cell damage resulting from them occur in various combinations. Thus, the micro organism, in its natural environment, is nearly permanently exposed to effects of stress and poor nutrition. It has to maximally adapt to constantly changing environmental conditions in order to successfully compete for nutrition and habitat and, thus, to give life to itself and its genus.

Such extremely high robustness enables the bacteria to pass both human and animal stomach - the organ designed to prevent, by using its acid environment, foreign micro organisms from entering the intestine - without any damage. One hundred percent of the strains contained in Dolgalet probiotics enter the intestine. This is explained by their ability to transform into inactive spores when the environment totally (or nearly totally) excludes the possibility of life. Bacillus subtilis, in the form of a spore nearly lacking any metabolic processes, has partially overcome even a spaceflight. In the second half of the nineties, German astronauts for the first time purposefully subjected bacteria to inanimate space conditions. Bacillus subtilis spores were loaded into Russian satellites. When totally unprotected, all the spores died quite quickly due to aggressive ultraviolet sunlight. However, when spores were mixed with meteorite powder or soil, a part of the micro organisms survived the spaceflight without any damage. In a cube, with a margin of only about 1 cm, nearly 100% of the micro organisms survived. This fact makes it possible to consider Bacillus subtilis as one of the most robust microorganisms (if not the most robust one) known by now. Also, as has been already described, Bacillus subtilis has a whole range of natural substances for self-protection and for neutralization of harmful effects of near environment.

The genome (i.e. the DNA sequence) of Bacillus subtilis has been completely decoded within an international genome-decoding project; it was shown to contain approximately 4,100 genes. At present, however, there are more than 3,000 different strains of Bacillus subtilis known worldwide. These strains have very similar properties, but, for the purposes of evaluation of their use, these strains should be considered individually. The first description was provided by Ehrenberg in 1835; he called it "Vibrio subtilis". In 1872, Kohn renamed "Vibrio subtilis" into “Bacillus subtilis”. The first documented medical use of this micro organism dates back to 1941. At that time, the sanitary unit of Wehrmacht African Corps, during its leap to Libya, used camel dung to treat the outbreak of diarrhea caused by Shigella sonnei in German soldiers. It should be remembered that, at that time, great shortage of drugs existed, and antibiotics were unavailable. Field studies showed that local population successfully treated the diarrhea by oral administration of fresh, warm camel dung. Therapeutic success was due to Bacillus subtilis that was present in large amounts in the dung. This is one of the documented examples of fantastic properties of this bacterial species. Unfortunately, neither these nor other incredible results of such therapy managed to compete successfully with the monopoly of antibiotics in the treatment of infectious diseases established since the discovery of penicillin in the beginning of forties. Except for small exclusions, they were completely forgotten. However, antibiotic resistance has become such a great problem that the near future can be expected to show, apart from the use of effective food additives, the return to the therapeutic methods that use effective probiotics.

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